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Caractérisation enzymatique des hémocytes d'huîtres plates, Ostrea edulis ArchiMer
Rousseau, Sabrina.
Depuis la fin des années soixante, la conchyliculture française doit faire face à des mortalités importantes d'huîtres plates, Ostrea edulis, causées entre autres, par le protozoaire, Bonamia ostreae, responsable de la bonamiose. Peu de moyens sont à la disposition des professionnels, vis-à-vis de cette maladie, pour protéger ces animaux élevés en milieu ouvert. Pour combattre la bonamiose et tenter d'apporter des solutions aux conchyliculteurs, IFREMER (Institut Français de Recherche pour l'Exploitation de la MER) a développé un programme de sélection pour obtenir des animaux résistants. Ces derniers sont obtenus en exerçant une pression de sélection par inoculation de parasites purifiés dans la cavité péricardique d'animaux anesthésiés. Ce processus se...
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Ano: 2001 URL: http://archimer.ifremer.fr/doc/00368/47919/47948.pdf
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Comet assay in phytoplankton as biomarker of genotoxic effects of environmental pollution ArchiMer
Akcha, Farida; Arzul, Genevieve; Rousseau, Sabrina; Bardouil, Michele.
The alkaline comet assay was tested on different microalgae: the clinofiagellates, Karenia mikimotoi and Alexandrium minuturn, and the diatom, Chaetoceros gracilis. The microalgae were exposed during their exponential growth to the model direct genotoxicant, hydrogen peroxide (1 h, 5 and 100 pM H2O2). Following H2O2 exposure, the comet assay was validated only for K. mikimotoi for which genotoxicity was observed from the lowest tested concentration of 5 pM with a concentration-dependent effect. C. gracilis was too small in size (4 mu m) to be correctly analysed. For A. minuturn, our lysis buffer was not strong enough to digest the cellulosic thecal plates. For K. mikimotoi, the comet assay was thus applied for the study of the genotoxic effects of...
Tipo: Text Palavras-chave: Comet assay; Genotoxicity; Pesticides; Phytoplankton.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4504.pdf
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Investigating the relationship between embryotoxic and genotoxic effects of benzo[alpha]pyrene, 17 alpha-ethinylestradiol and endosulfan on Crassostrea gigas embryos ArchiMer
Wessel, Nathalie; Rousseau, Sabrina; Caisey, Xavier; Quiniou, Francoise; Akcha, Farida.
Genotoxicity biomarkers are widely measured in ecotoxicology as molecular toxic endpoints of major environmental pollutants. However, the long-term consequences of such damage still have to be elucidated. Some authors have suggested that the accumulation of unrepaired DNA lesions could explain the embryotoxicity of certain chemical pollutants. As embryotoxicity exerts a direct impact on the recruitment rate, genotoxicity could be closely related to disturbances of ecological concern and produce a possible impact upon population dynamics. The aim of the present work was to study the genotoxicity and the embryotoxicity of three relevant pollutants for oyster embryos: the polycyclic aromatic hydrocarbon, benzo[a]pyrene (BaP), the synthetic estrogenic...
Tipo: Text Palavras-chave: 17 alpha ethinylestradiol; BaP; Endosulfan; DNA damage; Embryotoxicity; Pacific oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4058.pdf
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